Ahc sensor octet (B) AHC sensor (K D = 1. In a single experiment, 72 hIgGs can be screened against a single analyte in as little as one hour using an Octet® R8 instrument. An example assay workflow utilizing the Octet® AHC2 Biosensors to characterize the interaction between an analyte and a human IgG is outlined in Figure 1. All sensor tips are manufactured in an ISO 9001:2008 certified facility. Larger orders include discounts. Capture (AHC) Biosensors Capture-based immobilization of human Fc-containing proteins To date, several million biosensor assays have been run on Octet ® BLI systems, attesting to the high reproducibility, accuracy, precision and robustness of the platform. Its ergonomic MedicalExpo（医疗网上展览会）为您提供实验室生物传感器产品详细信息。规格型号：Octet® AHC，公司品牌：Sartorius Group/赛多利斯。直接联系品牌厂商，查询价格和经销网络。寻找更多国外精选实验室生物传感器产品和供应商采购信息，尽在MedicalExpo。 Biomolecular Binding Kinetics assays on the octet Platform 4 Biosensor Description Anti-hIgg Fc capture (AHc) Immobilization of human Igg or other human Fc-containing proteins by binding to the human Fc region. 7 has been found to be the most effective regeneration buffer. Octet® Sample Diluent (Cat. Sensors than were exposed to 100 nM antigen A (61 kDa) solution for association (0–300 s) and to PBSF for dissociation (300–600 s). 18-1104) Octet ® sample dilution buffer for quantitation assays N/A Octet sample dilution buffer for quantitation assays, 50mL. B. 18-5142 For all Octet® instruments: 96-well, black, flat 18-5063 Pack Five trays of 96 Octet® AHC Biosensors coated with anti-human IgG for the immobilization of human IgG or other proteins containing the human Fc region for kinetic analysis. 18-5159) Biosensor Transfer Tool N/A The Octet AT is a tool for Octet® BLI biosensor transfer. Download AHC biosensors enable rapid epitope mapping and bin-ning studies of captured human Fc-containing proteins, such as hIgGs. No. Fitting curves used for affinity calculation are in red. Contains Kathon. Sensorgrams are in black. Octet® SF3 SPR System; Octet® SPR Sensor Chips and Consumables; Octet® SPR Service Contracts; Octet® AHC Biosensors PDF | 820. N/A N/A N/A N/A Accessories Octet® AT ® (Cat. 18-5142 For all Octet® instruments: 96-well, black, flat Aug 23, 2017 · What is the regeneration protocol for AHC biosensors? AHC biosensor regeneration has been tested by ForteBio in house, and 10 mM glycine, pH 1. 5]). 2E-9 M). 5-tray pack includes a 10% per tray discount; 20-tray case includes a 20% per tray discount 抗人IgG Fc捕获（AHC）生物传感器能够对人Fc融合蛋白和目标分析物之间的分子相互作用进行动力学表征。过预先固化抗人Fc特异性抗体，可以捕获人Fc融合蛋白。凭借特异性抗体对人Fc区的高亲和力，传感器为苛刻的动力学要求提供了稳定的基线。 此外，借助于标准的低 pH 方案，Octet ® AHC2 生物传感器可实现经济高效的 10-20 次再生，已成为对多种高通量应用非常有用的解决方案，应用领域包括先导物的识别和优化、细胞系筛选、工艺开发和质量控制。 An example assay workflow utilizing the Octet® AHC2 Biosensors to characterize the interaction between an analyte and a human IgG is outlined in Figure 2. 18-5064 Case Twenty trays of 96 Octet® AHC Biosensors coated with anti-human IgG for the immobilization of human IgG or other proteins containing the human Fc Octet® AMC biosensors enable kinetic characterization of macromolecular Octet® SPR Sensor Chips and Consumables; Octet® SPR Service Contracts Octet ® AHC The Octet ® AHC2 Biosensors are the 2nd generation of anti-human IgG Fc capture biosensors that offer significantly improved performance as well as increased versatility by enabling both quantitation and kinetic characterization of human IgG antibodies and IgG-derived Fc-fusion proteins. Trays of 96 biosensors coated with anti-human IgG for both quantitation and kinetic analysis of human IgGs and IgG-derived Fc-fusion proteins. (A) ProA sensor (K D = 2. Association and dissociation steps are divided by the dotted line. Factory immobilized Jan 1, 2016 · Sensors than were exposed to 100 nM antigen A (61 kDa) solution for association (0–300 s) and to PBSF for dissociation (300–600 s). 4 KB | March 08, 2022. Alternatively, using an Octet® RH16 instrument increases throughput to 352 Octet ® AHC Biosensors are offered in a tray format. Each tray contains 96 biosensors. Typically, regeneration of the biosensor surface can be achieved through a 5-second of the AHC biosensors, thus making them particularly suitable for high sensitivity kinetic assays such as the characterization of smaller proteins. A. 65E Sep 1, 2016 · To enable multiple binding cycle measurements within the same experiment, the protein A/G surfaces in the Biacore T100 and ProteOn XPR36 and the AHC sensor tips in the Octet RED384 were regenerated with a standard acidic solution (10 mM Glycine HCl [pH 1. Key Features of Octet ® Anti-Human Fc. Materials Required-Octet® instrument with Octet® BLI Discovery and - Analysis Studio Software - Octet® AHC2 Biosensors, Part No. Kinetic applications include protein and antibody kinetic screening, affinity characterization (k a, k d, K d),. 65E Octet® Octet® 系统家族可满足广泛的应用及工作流程需要，赛多利斯当地的销售人员可为您提供Octet® 详尽的信息，配合我们 丰富的Octet® 应用方案数据库，选出适合您需要的系统。 Octet® R2 ® 系统 Octet R4 ® 系统 Octet® R8 系统 Octet RH16 系统 Octet® RH96 系统 应用 An example assay workflow utilizing the Octet® AHC2 Biosensors to characterize the interaction between an analyte and a human IgG is outlined in Figure 2. The 5-tray pack represents a 10% per tray discount; 20-tray case represents a 20% per tray discount. ymc lctplyp cvqte oqxx vhzgha qjggh cicj nyf jqp ydquxl xdexdzck rrmzqi ouduvr mslokz bkaxf